CST7 (gene)

Cystatin F (leukocystatin)

PDB rendering based on 2ch9.

Available structures
PDB	2ch9

Identifiers

Symbols

CST7; CMAP

External IDs

OMIM: 603253 MGI: 1298217 HomoloGene: 37832 GeneCards: CST7 Gene

Gene Ontology
Molecular function	• endopeptidase inhibitor activity • cysteine-type endopeptidase inhibitor activity
Cellular component	• extracellular region • cytoplasm
Biological process	• immune response • negative regulation of endopeptidase activity
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 20:
24.93 – 24.94 Mb

Chr 2:
150.4 – 150.4 Mb

PubMed search

[1]

[2]

Cystatin-F is a protein that in humans is encoded by the CST7 gene.^[1]^[2]^[3]

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. This gene encodes a glycosylated cysteine protease inhibitor with a putative role in immune regulation through inhibition of a unique target in the hematopoietic system. Expression of the protein has been observed in various human cancer cell lines established from malignant tumors.^[3]

References

^ Ni J, Fernandez MA, Danielsson L, Chillakuru RA, Zhang J, Grubb A, Su J, Gentz R, Abrahamson M (Oct 1998). "Cystatin F is a glycosylated human low molecular weight cysteine proteinase inhibitor". J Biol Chem 273 (38): 24797–804. doi:10.1074/jbc.273.38.24797. PMID 9733783.
^ Halfon S, Ford J, Foster J, Dowling L, Lucian L, Sterling M, Xu Y, Weiss M, Ikeda M, Liggett D, Helms A, Caux C, Lebecque S, Hannum C, Menon S, McClanahan T, Gorman D, Zurawski G (Aug 1998). "Leukocystatin, a new Class II cystatin expressed selectively by hematopoietic cells". J Biol Chem 273 (26): 16400–8. doi:10.1074/jbc.273.26.16400. PMID 9632704.
^ ^a ^b "Entrez Gene: CST7 cystatin F (leukocystatin)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=8530.

External Links

The MEROPS online database for peptidases and their inhibitors: I25.007

Brown WM, Dziegielewska KM (1997). "Friends and relations of the cystatin superfamily--new members and their evolution.". Protein Sci. 6 (1): 5–12. doi:10.1002/pro.5560060102. PMC 2143511. PMID 9007972. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2143511.
Saitoh E, Isemura S, Sanada K, et al. (1989). "Cystatin superfamily. Evidence that family II cystatin genes are evolutionarily related to family III cystatin genes.". Biol. Chem. Hoppe-Seyler 369 Suppl: 191–7. PMID 3202964.
Thiesse M, Millar SJ, Dickinson DP (1994). "The human type 2 cystatin gene family consists of eight to nine members, with at least seven genes clustered at a single locus on human chromosome 20.". DNA Cell Biol. 13 (2): 97–116. doi:10.1089/dna.1994.13.97. PMID 8179826.
Morita M, Yoshiuchi N, Arakawa H, Nishimura S (1999). "CMAP: a novel cystatin-like gene involved in liver metastasis.". Cancer Res. 59 (1): 151–8. PMID 9892200.
Morita M, Hara Y, Tamai Y, et al. (2001). "Genomic construct and mapping of the gene for CMAP (leukocystatin/cystatin F, CST7) and identification of a proximal novel gene, BSCv (C20orf3).". Genomics 67 (1): 87–91. doi:10.1006/geno.2000.6237. PMID 10945474.
Journet A, Chapel A, Kieffer S, et al. (2001). "Towards a human repertoire of monocytic lysosomal proteins.". Electrophoresis 21 (16): 3411–9. doi:10.1002/1522-2683(20001001)21:16<3411::AID-ELPS3411>3.0.CO;2-M. PMID 11079561.
Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20.". Nature 414 (6866): 865–71. doi:10.1038/414865a. PMID 11780052.
Nathanson CM, Wassélius J, Wallin H, Abrahamson M (2003). "Regulated expression and intracellular localization of cystatin F in human U937 cells.". Eur. J. Biochem. 269 (22): 5502–11. doi:10.1046/j.1432-1033.2002.03252.x. PMID 12423348.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Cappello F, Gatti E, Camossetto V, et al. (2004). "Cystatin F is secreted, but artificial modification of its C-terminus can induce its endocytic targeting.". Exp. Cell Res. 297 (2): 607–18. doi:10.1016/j.yexcr.2004.03.048. PMID 15212960.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Langerholc T, Zavasnik-Bergant V, Turk B, et al. (2005). "Inhibitory properties of cystatin F and its localization in U937 promonocyte cells.". FEBS J. 272 (6): 1535–45. doi:10.1111/j.1742-4658.2005.04594.x. PMID 15752368.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Schüttelkopf AW, Hamilton G, Watts C, van Aalten DM (2006). "Structural basis of reduction-dependent activation of human cystatin F.". J. Biol. Chem. 281 (24): 16570–5. doi:10.1074/jbc.M601033200. PMID 16601115.

PDB gallery

2ch9: CRYSTAL STRUCTURE OF DIMERIC HUMAN CYSTATIN F

CST7 (gene)

References

External Links

Further reading